Objective To explore M235 T polymorphism of angiotensinogen (AGT) gene and I/D polymorphism of angiotensin converting enzyme ( ACE ) gene in renin-angiotensin system,and their relationship with Henoch- Schonlein purpura ( HSP ) / Henoch - Schonleinpurpura nephritis ( HSPN ).Methods Insertion/deletion (I/D ) polymorphism in intron 16 of ACE gene and number two exon M235 T polymorphisms of AGT gene were analyzed in 90 patients with HSP/HSPN and 98 healthy adult controls,by using PCR-RFLP and PCR.Results ①The AGT genotype distribution was significantly different between HSP and normal control,and between HSPN and normal control ( P =0.008,P =0.002 ),but no difference was observed between HSP and HSPN ( P =0.180 ).The AGT gene TT genotype and T allele has a high risk for HSP and HSPN.②The ACE genotype distribution was significantly different between HSPN and normal control ( P =0.003 ),but no difference was observed between HSP and normal control,neither between HSP and HSPN( P =0.065,P =0.073 ).The ACE gene DD genotype and D allele has a high risk for HSPN.Conclusion AGT gene M235T polymorphism is associated with HSP/HSPN.ACE gene I/D polymorphism is associated with HSPN.%目的 探讨肾素-血管紧张素系统中AGT基因M235T和ACE基因I/D多态性与过敏性紫癜和过敏性紫癜性肾炎易感性的关系.方法 应用PCR和PCR - RFLP技术检测145例过敏性紫癜/过敏性紫癜性肾炎患者与172例正常对照组血管紧张素原基因第2外显子M235T多态性及血管紧张素转换酶基因第16内含子I/D多态性.结果 ①AGT基因型构成比在HSP组、HSPN组与正常对照组之间差异有统计学意义(P=0.008,P=0.002),但在HSP和HSPN之间差异无统计学意义(P =0.180).AGT- TT基因型和T等位基因携带者具有较高的患HSP、HSPN的风险.②ACE基因型构成比在HSPN组与正常对照组之间差异有统计学意义(P=0.003),但在HSP和正常对照组、HSP和HSPN之间差异无统计学意义(P=0.065,P=0.073).ACE - DD基因型和D等位基因携带者具有较高的患HSPN的风险.结论 携带AGT基因M235T多态性增加患HSP/HSPN的风险,携带ACE基因I/D多态性增加患HSPN的风险.
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