目的 研究慢性HBV感染者、健康人的外周血树突状细胞(DC)经HBsAg活化后免疫功能的差异.方法 从慢性HBV感染组及健康组外周血中培养扩增(以HBsAg刺激)DC和细胞因子诱导的杀伤细胞(CIK).用流式细胞仪检测DC、CIK的表型,用酶联免疫吸附法(ELISA)检测DC、CIK上清液中的白细胞介素-12(IL-12)的浓度,用CCK-8比色法测定DC共培养的CIK对HepG2.2.15细胞的杀伤活性.结果 经HBsAg致敏的健康组DC表面标志CD1a、CD80、HLA-DR及CD83阳性率明显高于慢性HBV感染组,差异具有统计学意义(P<0.01);慢性HBV感染组中经HBsAg致敏的DC表面标志阳性率与未经HBsAg致敏者相比差异无统计学意义(P>0.05).健康组DC经HBsAg致敏后诱导的CIK对HepG2.2.15细胞的杀伤率显著高于慢性HBV感染组(P<0.01).结论 慢性HBV感染组与健康组DC经HBsAg活化后,对HepG2.2.15细胞的免疫效应差异有统计学意义,健康组高于慢性HBV感染组.%Objective To study differences on immune effect of dendritic cell activated by HBsAg in healthy people and people with chronic HBV infective. Methods Dendritic cells(DC) and cytokine-induced killer(CIK) cells (activated by HbsAg) were cultured and amplified from peripheral blood of healthy people and people with chronic HBV infective. DC and CIK phenotype were detected by flow cytometry method. KLISA was used to detect the level of IL-12 in the upper liquid medium which DC-CIK cell mixed culture. The cell-killing activity of DC-induced CIK cell for HepG2. 2. 15 was measured by using CCK-8 colorimetric assay. Results In healthy people, DC was activated by HBsAg, the positive rate of four surface markers CD1a, CD80, HLA-DR and CD83 were significantly higher than those in people with chronic HBV infection(P<0. 01). In people with chronic HBV infection, there was no significant difference on DC surface markers between with or without HbsAg-pulsed(P>0. 05). In healthy people, the cell-killing rate of DC-induced CIK cell (HbsAg-pulsed) for HepG2. 2. 15 was significantly higher than those in people with chronic HBV infection(P<0. 01). Conclusion The immune effect of HBsAg pulsed for HepG2. 2. 15 cells are significantly different between healthy people and people with chronic HBV infective, the former is stronger than the latter.
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