定量ELISA法检测健康人血浆抗呼吸道合胞病毒IgG抗体的可行性研究

摘要

Objective To identify feasibility of a quantitative ELISA for detecting IgG antibody against respiratory syncytial virus (RSV) in healthy human plasma.Methods The quantitative ELISA was used to detect anti-RSA IgG in healthy human plasma.The positive plasma samples (anti-RSV titer ＞ 11 U/ml) were detected again by the quantitative ELISA.A neutralization assay was carried out meanwhile.Results In 3041 plasma samples detected,anti-RSV positive rates of initial and repeat detection were 5.4％ and 4.7％,respectively.The quantitative ELISA had good specificity and precision.The efficient detection rate of antiRSV positive plasma was 87.8％.Conclusion The quantitative ELISA can be applied to mass screening of anti-RSV IgG antibody in healthy human plasma.%目的 确认定量ELISA法检测健康人血浆抗呼吸道合胞病毒(respiratory syncytial virus,RSV) IgG抗体的可行性.方法 采用定量ELISA法检测健康人血浆,对初检的抗-RSV阳性血浆(抗-RSV效价＞11 U/ml)进行复检和中和试验.结果 检测3041份血浆,初检和复检的血浆抗-RSV阳性率分别为5.4％和4.7％.定量ELISA法的特异性和精密性均良好,抗-RSV阳性血浆的有效检出率为87.8％.结论 定量ELISA法可用于健康人血浆抗RSV IgG抗体的大规模筛检.