目的:研究六味地黄汤中芍药苷、薯蓣皂苷元、马钱苷、莫诺苷、桃叶珊瑚苷对小鼠T、B淋巴细胞增殖的影响.方法:制备脾淋巴细胞,MTr比色法检测不同浓度5种苷类单体单独作用及其与丝裂原( ConA、LPS)共同作用时对小鼠T、B淋巴细胞增殖作用的影响.结果:10-5M芍药苷能显著促进淋巴细胞增殖,对LPS诱导的B淋巴细胞增殖有明显的协同作用,10-7～10-6M芍药苷对ConA诱导的T淋巴细胞增殖有明显协同作用,与对照组比较有显著性差异(P＜0.01,P＜0.05);薯蓣皂苷元有一定促进淋巴细胞增殖作用,但与对照组比较无显著性差异;10-7～10-5M马钱苷能显著促进淋巴细胞增殖,10-7M马钱苷对LPS诱导的B淋巴细胞增殖有显著协同作用,随剂量增加,增殖作用反而降低;10-7～10-5M莫诺苷能显著促进淋巴细胞增殖,对LPS诱导的B淋巴细胞增殖有明显的协同作用,对ConA诱导的T淋巴细胞增殖有明显协同作用,与对照组比较有显著性差异(P＜0.01).10-7～10-5M桃叶珊瑚苷基本无促进淋巴细胞增殖作用,10-5M桃叶珊瑚苷对LPS诱导的B淋巴细胞增殖有明显的协同作用,10-7～10-5M桃叶珊瑚苷对ConA诱导的T淋巴细胞增殖有明显协同作用,与对照组比较有显著性差异(P＜0.01,P＜0.05).结论:六味地黄汤中5种苷类单体对T、B淋巴细胞均有不同程度增殖作用,莫诺苷作用最明显,马钱苷低剂量促进增殖,高剂量反而抑制.%Objective: The effects of five compounds ( paeoniflorin, diosgenin, loganin, morroniside and aucu-bin) in Liuwei Dihuang decoction on proliferative activities of mice splenic lymphocytes are investigated. Method: Paeoniflorin, diosgenin, loganin, morroniside and aucubin with or without concanavalin A (ConA) and lipopolysaccharide (LPS) were added into splenic lymphocyte. Effects of five compounds on the proliferative activities of T/B lymphocytes were measured by MTT colorimetric method. Results: Proliferative activities of cells that were treated with paeoniflorin (10-5M), loganin (10-7 ~10-5M) and morroniside (10-7 ~10-5 M) were promoted without ConA or LPS; LPS - induced proliferation of B lymphocytes was significantly promoted by paeoniflorin (10-5M), loganin (10-7M), morroniside (10-7 ~10-5M) and aucubin (10-5M); ConA - induced proliferation of T lymphocytes was markedly enhanced by paeoniflorin (10 -7 ~ 10-6 M) , morroniside (10~7 ~10-5M) and aucubin (10-7 ~10-3M), diosgenin played a certain role in promoting lymphocyte proliferation, but there was no significant difference. Conclusion; Paeoniflorin, diosgenin, loganin, morroniside and aucubin in Liuwei Dihuang decoction can enhance the proliferative activities of mice splenic lymphocytes to different degrees. The effect of morroniside on proliferative activity of lymphocytes is stronger than the effects of other compounds.
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