大蒜提取液与环丙沙星对铜绿假单胞菌生物被膜弹性蛋白酶影响的研究

摘要

目的:测定铜绿假单胞菌两个主要细胞外毒性因子弹性蛋白酶的活性,比较大蒜提取液与环丙沙星对其影响.方法:将大蒜分为40ug/mL,80ug/mL,100ug/mL三组,环丙沙星分为0.3ug/mL,O.5ug/mL,lug/mL三组及空白对照组,共8组.测定其对PA01和PAO-JP:的影响.结果:同一浓度大蒜液对PAOI弹性蛋白酶抑制幅度大于PAO-JP2,不同浓度环丙沙星的对PAO1,PAO-JP2弹性蛋白酶抑制幅度统计学显示无明显差异(P>0.05).结论:由此推测,大蒜液对QS系统有抑制作用,环丙沙星对QS系统无抑制作用.%Objective :To test the activity of the elastase of two extracellula cytotoxicity factor of Pseudomonas aeruginosa, and compare the influence of Garlic Extracts and ciprofloxacin to it.Methods :8 groups are divided into the garlic group(40ug/mL,80ug/mL, 100ug/mL);ciprofloxacin group(0.3ug/mL,0.5ug/mL, 1ug/ mL) and the blank group.Result:The inhibition of the garlic extracts to the elastase of PAO1 is higher than PAO - JP2 at the same concentration, while the inhibition of ciprofloxacin to the elastase of PAO1 and PAO - JP2 is not obviously different on the different concentration ( P ＞ 0.05 ).Conclusion: According to this, the garlic extracts has a suppressive function for QS system while ciprnfloxacin has not.