炮制前后淫羊藿对实验动物基础和能量代谢的影响

摘要

Objective:To compare the effect of crude and processing epimedium on basal and energy metabolism and to explore the alteration of medical nature of epimedium during the processing procedure.Method:30 KM mice were randomly divided into a blank group,a crude group and a processing group.The crude group and the processing group was given 175.5 mg/kg once a day,and the blank group was given the same volume of distilled water instead.After seven days,the heat output(HEAT),energy transformation ratio (RER),the amount of O2inhalation and CO2 exhalation were measured by the Comprehensive Laboratory Animal Monitoring System.30 SD rats were randomly divided into a blank group,a crude group and a processing group,respectively,and were given the distilled water and the corresponding drug 175.5 mg/kg once a day.After seven days,the contents of TT3,TT4,TSH,TAOC,SDH and ATPase in serum were determined to explore the effect of crude and processing epimedium aqueous solution on basal and energy metabolism of laboratory animals,and the medical nature of epimedium was studied.Results:Results of mice basal metabolic test suggested that compared with those in the blank group,the body weight and ear temperature of the processing group were increased,and the coefficiency of autonomic activity in the crude group was significantly decreased (P ＜ 0.05),and the calorie production in the processing group was significantly increased (P ＜0.01),and the amount of O2inhalation and CO2 exhalation of crude group was significantly decreased (P ＜ 0.01).Results of rat basal metabolic test demonstrated that the level of TT4 and TSH was significantly decreased (P ＜ 0.01) and did not have any impact on the level of TT3 in crude group.There was no significant difference in TT3,TT4 and TSH contents in the processing group (P ＜ 0.01).In addition,the contents of TAOC,SDH and ATPase were significantly different between the crude group and the processing group (P ＜ 0.01).Conclusion:The processing epimedium can promote the basal and energy metabolism of laboratory animals and the processing procedure may alter the medical nature from cold to lukewarm.%目的:通过研究淫羊藿炮制前后对实验动物基础和能量代谢影响,探求炮制作用对淫羊藿药性的影响.方法:KM小鼠30只随机分成空白组、生品组和炮制组,生品组和炮制组分别给予相应药物灌胃(175.5 mg/kg),空白组用等体积蒸馏水代替,连续7天后,通过实验动物监测系统对给药前后动物产热量(HEAT)、能量转化率(RER)、O2吸入量和CO2呼出量、自主活动次数进行检测.SD大鼠30只随机分为空白组、生品组和炮制组,分别给予相应药物灌胃(175.5 mg/kg),连续7天后测定血清中与物质能量代谢相关指标TT3、TT4、TSH、TAOC、SDH、ATPase的含量,从而探讨炮制前后淫羊藿水煎液对实验动物基础代谢和能量代谢的影响来进行药性研究.结果:从小鼠基础代谢实验结果得到,与空白组比较,炮制组的体质量和耳温均有所升高,生品组的自主活动系数明显下降(P＜0.05),炮制组小鼠产热量显著升高(P＜0.05),生品组小鼠氧气吸入量与二氧化碳呼出量均显著降低(P＜0.01);大鼠能量代谢实验结果得到,生品组可显著降低TT4与TSH的水平(P＜0.01),对TT3含量无影响,炮制组对TT3、TT4、TSH含量均有显著性升高(P＜0.01).此外,生品组和炮制组对TAOC、SDH、ATPase含量均有显著性差异(P＜0.01).结论:淫羊藿炮制后对实验动物的基础代谢与能量代谢均有促进作用,且炮制淫羊藿的药性由寒性转为温性可能是由于炮制所致.