自身免疫性肝炎患者外周血中髓系来源抑制性细胞频率变化

摘要

Objective To investigate the frequency of myeloid-derived suppressor ceils (MDSCs) in peripheral blood in patients with autoimmune hepatitis (AIH) and its correlation with disease progression. Methods Peripheral blood samples from 22 AIH patients and 24 healthy controls were collected. The frequency of MDSCs was analyzed using flow cytometry and its correlation with liver biochemical indicators was evaluated as well. Results The frequencies of MDSCs in non-cirrhotic AIH patients were higher than those in cirrhotic AIH patients and in healthy controls, and the differences between them were significant (P＜0.05). But no difference was found between cirrhotic AIH patients and healthy controls (P＞0.05). The frequencies of MDSCs in peripheral blood were positively correlated with ALT and AST in all AIH patients. Conclusions In non-cirrhotic AIH patients, the peripheral frequencies of MDSCs increase correspondingly and autoimmune responses are inhibited. However, a variety of causes lead to the decrease in the number of MDSCs in cirrhotic AIH patients, thus accelerating the progress of liver injury and liver cirrhosis.%目的 探讨自身免疫性肝炎(autoimmune hepatitis,AIH)患者外周血中髓系来源抑制性细胞(myeloid-derived suppressor cells,MDSCs)频率及其与疾病的关系.方法　采集22例AIH患者和24例健康对照者外周血,用流式细胞仪分析MDSCs频率,对患者MDSCs频率与肝脏生化指标进行相关性分析.结果　非肝硬化组外周血MDSCs频率明显高于肝硬化组和健康对照组,差异有统计学意义(P<0.05).肝硬化组与健康对照组相比,差异无统计学意义(P>0.05).所有患者MDSCs频率与ALT和AST水平成正相关.结论 在AIH非肝硬化期,外周血MDSCs频率呈反馈性增高,抑制体内自身免疫反应;在AIH肝硬化期,各种原因致使MDSCs数量下调,加速肝细胞损伤和肝硬化进程.