首页> 中文期刊> 《工业微生物》 >不同增菌和培养条件下沙门氏菌检出效果的比较

不同增菌和培养条件下沙门氏菌检出效果的比较

         

摘要

By adding low levels of Salmonella typhimurium and interfering bacteria (Citrobacter freundii and Proteus mirabilis) to samples of chicken essence powder, the different plate performances were compared. The comparisons were among 3 different types of selective enrichment broths, 4 different culture periods and 4 selective plates. Methods based on the testing standard of GB4789.4-2016 and microbiological examination of nonsterile products: Tests for Specified Microorganisms. As a result, the particular culturing procedure with distinct advantage was obtained as follows: started with pre-culture in RV (Rappaport Vassiliadis Salmonella Enrichment Broth) or TTB (Tetrathionate Broth Base enrichments) for 18 h ~ 20 h, then followed by a selective plate culturing of Salmonella Chromogenic Medium or BS (Bismuth Sulfite Agar).%通过在鸡精样本中添加低含量的鼠伤寒沙门氏菌和干扰菌(弗氏柠檬酸杆菌和奇异变形杆菌),参考国标和美国药典中沙门氏菌的定性方法,研究比较了3种选择性增菌液、4个培养时间段以及4种选择性平板对于沙门氏菌的不同检出效果.结果表明,在RV肉汤及四硫磺酸钠煌绿增菌液(TTB)中培养18 h~20 h,使用沙门显色培养基及亚硫酸铋琼脂(BS平板)进行选择性培养沙门氏菌的检出效果最好.

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