Streptomyces sp. FIM-16-06产他克莫司摇瓶发酵条件的优化

摘要

采用单因素试验和正交试验研究发酵培养基组成,优化Streptomyces sp.FIM-16-06产他克莫司的发酵条件,探讨摇瓶发酵的主要影响因子初始pH、装液量、转速等发酵参数的影响.确定了适宜的发酵培养基和发酵参数:6.0％玉米淀粉、2.0％黄豆粉、2.0％葡萄糖和0.5％玉米浆,初始pH 7.5,装液量50 mL/500 mL三角瓶,种子菌龄48 h,接种量10％,摇床转速250 r/min,发酵温度27℃,发酵周期7 d.优化后的发酵水平较原生产工艺提高60％以上.他克莫司的优化发酵工艺为其工业化生产奠定了基础.%In order to optimize the fermentation conditions for tacrolimus-producing strain Streptomyces sp. FIM-16-06, the single factor experiments and orthogonal experiments were adopted to investigate the liquid fermentation medium and culturing parameters of Streptomyces sp. FIM-16-06. The results revealed that the appropriate fermentation medium composition and optimal fermentation parameters were determined as follows: fermentation medium containing corn starch6.0％, glucose 2.0％, soybean flour 2.0％ and corn steep liquor 0.5％, while the initial pH 7.5, filling volume 50 m L/500 m L shake flask, cultivation time of seed 48 h, inoculum size 10％, shaker''s rotating speed 250 r/min, fermentation temperature 27 ℃ and fermentation time 7 d. The tacrolimus yield of Streptomyces sp. FIM-16-06 under the optimized fermentation conditions was 60％ higher than that of the original. The optimal fermentation conditions of Streptomyces sp.FIM-16-06 laid the foundation for further industrial fermentation.