金属离子和氨基酸对Eupenicillium sp.E-UN41生物合成咪唑立宾的影响

摘要

By adding inorganic salts and amino acids in shake flask fermentation, the effects of metal ions and amino acids on the cell growth and the secondary metabolite mizoribine ( MZ) production by Eupenicillium sp. E-UN41 were investigated. Results showed that MZ production could be improved from 5% to 15% by addition of MgSO4 , NaCl, MnSO4 , KNO3and CaCO3 with proper concentration in the culture medium during fermentation. MZ production could increase 94% by adding 1.0% L-histidine to the medium. Some amounts of L-glycine or L-aspartic acid added to the medium could increase MZ production, but L-arginine had the evident inhibition effect on MZ production. It established the foundation for producing MZ on industrial scale.%为考察金属离子和氨基酸对Eupenicillium sp.E-UN41菌体生长及其产生的次级代谢产物咪唑立宾(MZ)的生物合成影响,在培养基中添加了无机盐和氨基酸.结果表明在发酵培养基中添加适量的镁、钠、锰、钾、钙等金属离子可提高咪唑立宾产量5％～15％,添加1.0％L-组氨酸,MZ产量提高94％.添加适宜的L-天门冬氨酸和L-甘氨酸对产MZ亦有促进作用,而L-精氨酸明显抑制Eupenicillium sp.E-UN41生物合成MZ.本研究为咪唑立宾发酵的产业化奠定了基础.