黄海海域沉积物和庐山土壤样品中放线菌的分离、活性及生物多样性研究

摘要

Using uniform design method,the optimal pretreatment conditions were the heat treatment at 50 ℃ for 20 min, no ultrasonic treatment and phenol addition. Under the optimal conditions,86 strains and 11 strains of actinomycetes were isolated from marine sediments of the Yellow Sea and Lushan soil samples,respectively. Based on molecular identifica-tion,86 actinomycetes of marine sediments belonged to 3 different genera and 11 strains of Lushan soil samples belonged to 4 different genera. Antimicrobial activities of the 97 isolated strains showed that 18. 5% of strains had inhibition zone to Escherichia coli,and 7. 2% of strains had anti-Bacillus subtilis activity,but all strains could not inhibit against Saccharo-myces cerevisiae.%本研究采用均匀实验设计优化预处理条件来分离黄海海泥和庐山土壤样品中的放线菌。通过均匀实验得到最优的预处理条件为湿热50℃处理20 min，不进行超声处理以及添加苯酚。经过形态排重后，从海泥和庐山样品中分离得到86株和11株不同表型的放线菌。通过进一步的分子生物学鉴定，海泥中的86株放线菌分别属于3个不同的属，而庐山样品的11株分别属于4个不同的属。对这97株放线菌进行抗菌实验发现，18．5％的菌株对大肠杆菌有抑菌活性，7．2％的菌株对枯草芽孢杆菌有抑菌活性，但对酿酒酵母都无抑菌活性。