为获得对水稻纹枯病有生防效果的拮抗细菌,从野外水稻田采集的水稻根际土壤样品中分离得到生防细菌菌株5株,以水稻纹枯病菌为靶标菌株,采用划线平板对峙法筛选出细菌菌株B-2对病原菌有较强的拮抗作用,采用纸片扩散法测定表明B-2对水稻纹枯病菌菌丝生长有抑制作用,结合16S rDNA序列分析,B-2鉴定为短小芽孢杆菌(Bacillus pumilus).抑菌试验结果表明,B-2对水稻纹枯病菌的抑菌活性为85.33％,能够明显抑制病原菌的生长.该研究结果对水稻病害的生物防控、绿色防控提供了科学依据.%To obtain antagonistic bacteria for biocontrol of rice sheath blight, five bacterial strains were isolated from the samples taken from different paddies and plants. The strain B-2 with strong antagonistic activities and growth inhibition to Rhizoctonia solani were selected by using the underlined plate confrontation method and Kirby-Bauer method. In combination with 16S rDNA sequence analysis, B-2 was identified as Bacillus pumilus. The results showed that the strain B-2 antagonistic bacteria had great effect to pathogenic bacteria, with the efficacy having 85％ in laboratory. The results of this study provide a scientific evidence for the prevention and control of rice diseases.
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