辣椒叶片总RNA快速提取

摘要

Trizol extraction method was modified to extract total RNA from Capsicum annuum leaves. The result of agar gel electrophoresis, ultraviolet ray photometer and RT-PCR showed that the total RNA obtained by modified Trizol method was of high quality, and suitable for downstream applications.%采用改良的Trizol法对辣椒(Capsicum annuum)叶片的总RNA进行了提取,利用琼脂糖凝胶电泳、紫外分光光度法、RT-PCR进行RNA纯度、完整性检测.结果表明,Trizol法提取可获得较高质量的辣椒叶片总RNA,能满足后续的研究需要.