一株产甘草酸内生真菌的分离及代谢产物分析

摘要

采用组织块法从采自内蒙古鄂尔多斯市的甘草根部分离纯化内生真菌,经液体发酵培养,抽提发酵粗产物,以甘草酸为标准品采用LC-MS法和HPLC法对这些真菌的代谢产物进行筛选,获得1株产甘草酸的内生菌,通过形态学研究初步确定该菌株为镰孢霉属.%A strain of glycyrrhizic acid-producing endophytic fungi was obtained from the rats of licorice in Erdos city of the Inner Mongolia autonomous region by tissue culture method. Then the purified endophytic fungal strains were fermented. The glycyrrhizic acid in the fungal extract was extract and confirmed by HPLC and LC-MS by comparison with glycyrrhizic acid standard. The strain B12 that was found as the glycyrrhizic acid-producing andophytic fungus was grouped into Fusarium based on the morphological traits.