盐胁迫对拟南芥种子萌发的影响

摘要

In order to study the influence of salt stress on seeds germination of Arabidopsis ttudiana (L.) Heynh., Columbia wild-type A. thaliana seeds were used as materials and inoculated into MS basic medium with 50, 100, 150, 200 mmol/L NaCl, 50, 100, 150, 200 mmol/L KC1, and 100, 200, 300, 400 mmol/L mannitol under the sterile conditions. Statistics were carried out for the seed germination rate for seven days. Results showed that when the concentration of NaCl, KC1, mannitol was 50, 50, 100 mmol/L, respectively, the seed germination rate was not significantly different from the control. When the concentration of NaCl, KC1 and mannitol reached to 150, 150 and 300 mmol/L, the seed germination rate was significantly lower than the control. With the increase of salt concentration in medium, the inhibitory effects on A. thaliana seeds germination and seedling growth were increased.%为了研究盐胁迫对拟南芥种子萌发的影响,以哥伦比亚野生型拟南芥种子为试验材料,在无菌条件下将拟南芥种子分别点种在添加50、100、150、200 mmol/L的NaCl和KCl及100、200、300和400mmol/L甘露醇的MS基本培养基中进行培养；然后对拟南芥种子的萌发情况进行统计,连续统计7d.结果表明,当NaCl、KCl和甘露醇浓度为50、50、100 mmol/L时,种子萌发率与没有盐胁迫的对照无显著差异;当NaCl、KCl和甘露醇浓度分别达到150、150和300 mmol/L时,种子萌发率均显著低于对照.随着培养基中盐浓度的提高,拟南芥种子萌发和幼苗生长所受到的抑制作用不断增强.