提取板栗DNA的CTAB法和SDS法的比较

摘要

Genomic DNA of chestnut (Castanea mollissima BL.) was extracted by CTAB method and SDS method. The DNA extracted was tested by agarose gel electrophoresis and spectrophotometry, and then double digested by EcoR I and Mse I for AFLP. The results showed that the DNA extracted by both methods was with good quality and high purity while DNA extracted by SDS method was with higher quality and purity compared with CTAB method.%分别采用十六烷基三乙基溴化铵(CTAB)法和十二烷基磺酸钠(SDS)法提取板栗(Castanea mollissima BL.)基因组DNA,取适量DNA进行琼脂糖凝胶电泳,并利用紫外可见分光光度计测定所提取的DNA在260和280 nm的吸光度及其比值,利用Eco R Ⅰ和Mse Ⅰ双酶切后进行随机扩增长度多态性(AFLP)分析.结果表明,两种方法均能从板栗中提取出一定质量的、比较完整的、纯度比较高的DNA,SDS法提取的板栗DNA的纯度更高、质量更好.