庐山楼梯草总黄酮提取工艺的优化

摘要

为研究庐山楼梯草(Elatostema stewardii Merr.)总黄酮的提取工艺,以乙醇溶液为溶剂,在单因素试验基础上利用响应曲面法的中心组合设计,对庐山楼梯草总黄酮提取工艺参数进行优化分析.结果表明,提取庐山楼梯草总黄酮的最优工艺条件为乙醇体积分数55％、提取温度80℃、液料比37∶1 (V/m,mL∶g)、提取时间2.4 h、提取2次,该条件下庐山楼梯草总黄酮提取率为5.14％.%Total flavonoids were extracted from Elatostema stewardii Merr. using alcohol as solvent. Single factor tests as well as response surface methodology based on a central composite design was applied to optimize the extraction process. The results showed that the optimized extraction conditions were volume fraction of alcohol solvent, 55%; extraction temperature, 80℃; solvent to material ratio, 37:1 (V/m,mL:g) ; extraction time, 2.4h; and extract for twice. Under these extraction conditions, the extraction yield could be up to 5.14%.