绿色木霉外源基因表达系统的构建

摘要

利用PCR方法克隆瑞氏木霉(Trichoderma reesei)外切葡聚糖纤维二糖水解酶Ⅰ基因(cbhⅠ)启动子序列,以pSK载体为骨架,构建了pSK-cbh Ⅰ-GFP-hph表达载体,并成功转入到绿色木霉(T.viride)Sn-9106中.通过对绿色木霉Sn-9106进行遗传改造,为纤维素酶基因在木霉中同源重组表达提供遗传转化平台.%Promoter of cellobiohydrolase Ⅰ gene (cbh Ⅰ) of Trichoderma reesei was cloned by PCR.The expression vector pSK-cbh Ⅰ-GFP-hph was constructed with pSK vector as backbone.Then it was introduced into the strain of T.viride Sn-9106 by transformation.The genetic modification of T.viride Sn-9106 could provide genetic transformation platform for homologous recombination expression of cellulase gene in T.spp..