TLR4能识别内毒素脂多糖(LPS),在介导革兰氏阴性菌及其LPS的宿主反应中发挥重要作用,是研究肉兔抗病力的重要候选基因.为了研究TLR4在不同肉兔品种的突变情况,设计引物对2个不同肉兔品种的12个个体的TLR4部分编码区进行序列扩增,并比较其序列.结果表明,共检测到3个SNP位点,均为非同义突变. 2个非同义位点(G628T和C653A)位于预测蛋白的亮氨酸富集区(LRR)区域. LRR区域发生的非同义突变, 尤其是亮氨酸的改变和氨基酸极性的改变可能会影响TLR4对病原的识别,这些SNP位点将有助于肉兔抗病分子标记的研究.%TLR4 could detect endotoxin by identifying lipopolysaccharide (LPS) and play a key role in host defense mediated by Gram-negative bacteria(LPS),therefore, TLR4 is a significant candidate gene for rabbit diseases resistance research. To i-dentify nucleotide polymorphisms between two different breeds we designed primer pairs that amplified fragments of coding se-quence (CDS) of 12 individuals, and compared the sequences. Comparative sequence analysis indicated that 3 SNPs were i-dentified, all were non-synonymous SNPs. Two nonsynonymous SNPs (G628T and A653C) were located in the LRR domains of the predicted protein. The nonsynonymous SNPs in the LRR domains, especially related to leucine residues, may dramati-cally alter the ability to identified extracellular pathogens. The identification of these polymorphisms reported here increases the resource of genetic markers useful for association analyses with disease resistance and provides an important theoretical basis for disease resistant breeding of meat rabbits.
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