In vitro method for differences of research in the processing proteolu00cdtico between two or more different samples, the use of the same, kit of reagents and devices for the analysis of two samples of protein using the if rotulau00c7u00c3o and analysis by multiplexau00c7u00c3o samples If rotulau00c7u00c3o protein using double.

摘要

The present invention relates to the simultaneous analysis of samples for determining differential proteolytic processing based on isotopic labelling of N-terminal peptides, wherein the isotopic labelling is achieved by incorporation of 18O during enzymatic proteolysis.