采用正交试验分析了不同环境条件对念珠藻地木耳(Nostoc commune Vauch)生物量和多糖分泌的影响.结果表明,在适宜的温度、光强、盐度和UV-B处理对地木耳的生长、胞外多糖分泌及其抗氧化能力有明显的促进作用.培养条件为光照强度100 μmol∕(m2?s)、温度30℃、NaCl浓度0 mmol∕L和UV-B辐射强度10 kJ∕(m2?d)时,地木耳有最大的相对生长率;光照强度200 μmol∕(m2?s)、温度35℃、NaCl浓度40 mmol∕L和UV-B辐射强度20 kJ∕(m2?d)时,胞外多糖的分泌强度最高,温度和UV-B处理分别对相对生长率和胞外多糖含量的提高有显著效果.%Impact of different environmental factors on growth and polysaccharides characteristics of Nostoc commune Vauch from Shenzhen seashore were analysed by orthogonal experiment. The results showed that under the appropriate conditions of temperature,illumination intensity,inorganic salt and UV-B treatment,the relative growth rate (RGR),the content and the anti-oxidant capacity of extracellular polysaccharides were significantly stimulated. The culture conditions were that 30℃ tempera-ture,0 mmol∕L NaCl,100 μmol∕(m2?s) illumination intensity and 10 kJ∕(m2?d) UV-B treatment,the relative growth rate was the highest. When the conditions were that 35℃ temperature,NaCl concentration at 40 mmol∕L,illumination intensity at 200 μmol∕(m2?s) and UV-B treatment at 20 kJ∕(m2?d),the extracellular po1ysaccharides concentration were the highest. The most significant factor for the relative growth rate(RGR) and EPSs were temperature and UV-B treatment,respectively.
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