原子吸收光谱法检测蔗汁中铁和锌含量的方法研究

摘要

建立一种准确、简单易行且适合大批量检测甘蔗蔗汁中铁(Fe)和锌(Zn)含量的方法,是筛选、培育和改良甘蔗新品种富铁和富锌的关键.试验基于原子吸收光谱法,经改进消化处理条件,形成了一种湿化-原子吸收光谱法检测甘蔗蔗汁Fe 和Zn 含量,主要用于甘蔗杂交育种中Fe 和Zn 含量的检测,用以开展"富铁甘蔗"和"富锌甘蔗"品种选育,旨在为甘蔗品质育种提供检测技术支撑.用体积比为7:2 的混合酸(硝酸:高氯酸)作为消解剂、同时通过控制消化温度等条件来消解处理甘蔗汁样,然后进行火焰原子吸收光谱法测定.结果表明,该方法的检出限分别为0.08 g∕kg 和0.042 g∕kg,相对标准偏差分别为3.93%和4.41%,加标回收率在98.8%~104.0%;此方法简便、准确、灵敏度高、重复性好、回收率高,适合于甘蔗汁中Fe 和Zn 的检测.%The significant characteristics of sugarcane cross-breeding are lots of materials and workload. The key work that screening,cultivation and improvement of sugarcane varieties which rich in Fe and Zn are the establishment of the methods, which could be an accurate and simple method and suitable for mass inspection. For the technical guidance of sugarcane quali-ty breeding, a method of determining the Fe and Zn in the sugarcane juice with Flame Atomic Absorption Spectromet were developed. Under the optimized conditions, the detection limits of the method for Fe and Zn were 0.08 g∕kg and 0.042 g∕kg, resjpectively,and the spike recoveries in sugarcane juice were in the range of 98.8%~104.0% with relative standard deviations of 3.93% and 4.41%. This method was easy to get,accurate and higher sensitivity,good recovery and respeatability,and suit-able for determining Fe and Zn in the sugarcane juice.