农杆菌介导转基因植物T-DNA的整合方式

摘要

农杆菌介导的遗传转化已被广泛应用于植物转基因研究.作为外源基因的载体,农杆菌T-DNA片段在植物基因组中的整合方式,不仅影响外源基因的整合效率及稳定性,还会影响外源基因的表达特性.文章就农杆菌介导的T-DNA整合的两种主要模式、规律及相关研究手段进行综述,为农杆菌介导的转基因及T-DNA插入突变等相关研究提供借鉴.%The genetic transformation mediated by Agrobacterium tumefaciens has been widely applied to research of transgenic plants. As the vector of the exotic genes, the integration patterns of T-DNA fragments affects not only transformation efficiency and stability, but also expression properties of the transgenes. This review summaries the two major patterns and the rules of T-DNA integration in Agrodacterim-mediated transformation, rules of T-DNA mediated by Agrobacterium tumefaciens, as well as research tools for flanking sequence amplification. It is attempted to provide references for researches on transformation and T-DNA integration mutation mediated by Agrobacterium tumefaciens.