干旱胁迫条件下马铃薯耐旱品种宁蒗182叶片蛋白质组学分析

摘要

开展马铃薯抗旱分子机理的研究对培育马铃薯抗旱品种,减少干旱造成的损失至关重要.文章利用双向 电泳技术对云南地方耐旱马铃薯品种宁蒗182 在干旱与正常处理条件下叶片表达差异蛋白质组进行对比研究.经电泳图谱分析和MALDI-TOF-TOF/MS 质谱鉴定获得12 个表达差异蛋白点,并进行了功能分类.结果发现,在差异蛋白中具有保护马铃薯光和系统以及线粒体正常运转的酶类;调节该植株对环境胁迫响应的信号传导 以及调控其组织内N、C 运输系统的功能蛋白,这些蛋白在受到干旱胁迫时表达量均升高.这一结果揭示出该 类蛋白是马铃薯在干旱条件下产生的耐受相关蛋白.文章为阐释马铃薯抗旱品种通过多种路径和水平的调控提 高其抗性的分子机理提供了理论依据.%It is utmost important to cultivate potato variety with drought resistance to reduce arid loss by research progress on potato drought resistance molecular mechanism. A comparative study with differences in protein group analysis of potato drought resistance variety in Ninglang 182 leaves was carried out by using two-dimensional gel electrophoresis during drought and normal processing conditions.There were 12 differentially expressed protein spots identified by Electro-rnphoresis and MALDI-TOF-TOF/MS analysis. The function classification of these proteins results that the potato varieties to drought tolerance could be improved through the protection of photosynthesis and mitochondria, regulation of the signal transduction induced under environmental stress and regulation of plant tissue N and C transport system, these proteins expression were increased under drought.The results showed that these proteins are the drought resistance associated proteins of potato variety in Ninglang 182.This study provided a theoretical basis of the molecular mechanism of improving drought tolerance in order to expound the potato drought resistance variety through multiple paths and the level regulation.