目的 建立高效液相色谱-蒸发光散射检测(HPLC-ELSD)法,测定并比较天然牛黄与酶促牛黄中胆固醇的含量.方法 色谱柱为Diamonsil C18柱(250 mm×4.6 mm,5 μm);以乙腈-异丙醇(1:1)为流动相,流速1.0 mL·min-1;漂移管温度67.5 ℃,气体流速1.7 L·min-1.结果 胆固醇浓度在4.0～20.0 μg·mL-1范围,浓度对数(logC)与峰面积对数(logA)呈良好的线性关系logA＝1.541 2logC-2.525 (r＝0.999 2);低、中、高浓度平均回收率分别为98.41%,100.04%,96.46%,RSD分别为0.63%,1.67%,1.03%.结论 该法简便、准确,专属强,可用于牛黄、牛黄替代品及其制剂的质量控制.%Objective To establish a HPLC-ELSD method for determining cholesterol and making a comparation of which between two kinds of calculus bovis. Methods A C18( 250 mmx4. 6 mm,5 |xm )column was used with a mobile phase as acetonitrile- Isopropanol (1:1 )flowing at 1. 0 mL ? Min"1. The temperature of drift tube was 67. 5 °C , and the gas flow rate was 1. 7 L ? Min"1. Results The cholesterol was in a good linear relationship in the range of 4. 0-20. 0 jjtg ? mL'1 , logA = 1. 541 21ogC-2. 525 ( r = 0. 999 2 ). The average recovery was 98. 41% , 100. 04% , 96. 46% , respectively, and RSD was 0.63% ,1.67% ,1.03% ,respectively. Conclusion The method is simple, accurate, specific and can be used for the quality control of cholesterol in natural calculus bovis, succedaneums and preparations.
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