肿瘤坏死因子α对人肝癌细胞株SMMC-7721体外侵袭能力的影响

摘要

目的 探讨肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)对人肝癌细胞株SMMC-7721体外侵袭能力的影响及其相关机制.方法 不同浓度TNF-α作用于人肝癌细胞株SMMC-7721后,采用Transwell试验检测细胞侵袭能力的改变,采用划痕标记荧光传输试验显示缝隙连接细胞通讯功能的变化,采用Western blot法检测连接蛋白32(Cx32)在细胞膜的表达.结果 不同浓度TNF-α作用于人肝癌细胞株SMMC-7721后,Transwell试验显示细胞侵袭能力较对照组提高,且随TNF-α浓度增加而升高.同时,缝隙连接细胞通讯功能减弱,细胞膜上Cx32的表达减少.结论 TNF-α可能通过影响Cx32在细胞膜的表达及细胞通讯促进人肝癌细胞株SMMC-7721的侵袭能力.%Objective To investigate the effect of TNF-α on the invasion of human hepatocellular carcinoma cell line SMMC-7721. Methods SMMC-7721 were treated with TNF-α of different concentrations for 24 h. The invasion of cells were detected hy Transwell assay. The gap junction intercellular communication between cells were detected by scrape-loading and dye transfer assay. The expression level of connexin 32 ( Cx32 ) on membrane was analyzed by Western blot. Results Compared with the control, cell invasion was enhanced by TNF-α in a dose-dependent manner. While the gap junction intercellular communication was weakened and Cx32 expression on membrane was decreased. Conclusion TNF-α may promote the invasion of human hepatocellular carcinoma cells through Cx32 and gap junction.