Objective To investigate the effect of serum containing matrine on the proliferation of human hepatoma cell line SMMC-7721,provide scientific evidence for liver cancer therapy with matrine. Methods Kunming mice were given matrine by gavage and then the blood was collected to prepare serum containing matrine. Human hepatoma cell line SMMC-7721 and mice serum containing matrine were used the research subject and the research drug,respectively. Serum containing matrine on the pro-liferation inhibitory activity of the tumor cells were determined by using MTT assay. It was observed that the effects of different dosage of administration and different culture time on the inhibitory activity. Results Serum containing matrine had different de-grees of inhibition on SMMC-7721. As compared with the cell control group and the normal saline group (negative control group) , there was significant difference the inhibitory activity(P<0.05or P<0.01). There was no significant difference when serum containing matrine of the high dosage at concentration of 40% were compared with serum containing 5-fluorouracil (5-Fu) group (positive control group)at the same concentration (P>0.05). And the inhibitory activity showed obvious dose-effect relationship. When serum containing matrine was cultured with the tumor cells for 24 and 48 hours,the inhibitory activity was better than for 72 hours. Conclusion Serum containing matrine has good effects of anti-hepatoma in vitro.%目的：探讨苦参素含药血清对人肝癌细胞株SMMC-7721增殖活性的影响，为苦参素应用于肝癌的治疗提供科学依据。方法昆明种小鼠灌胃给药，采血并制备含药血清。以SMMC-7721细胞株为研究对象，以小鼠含药血清为药物，应用MTT显色法分析苦参素含药血清对瘤细胞的抑制作用，观察不同给药剂量及不同培养时间对抑制作用的影响。结果苦参素含药血清对SMMC-7721细胞具有不同程度的抑制作用。与细胞对照组和生理盐水组（阴性血清对照组）比较，差异显著（P<0.05或P<0.01）；高剂量40%浓度的苦参素含药血清组与5-氟尿嘧啶（5-Fu）含药血清组（阳性血清对照组）比较，其抑制作用无明显差异（P>0.05)。含药血清对瘤细胞的抑制作用呈一定的量效关系。含药血清与瘤细胞共同培养24h和48h的抑制作用强于72h。结论苦参素含药血清具有良好的体外抗肝癌的药效作用。
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