目的 探讨黄芩苷对过氧化氢(H2O2)诱导的SH-SY5Y细胞损伤的Bcl-2和Bcl-xL mRNA表达的影响.方法 建立人神经母细胞瘤SH-SY5Y细胞的体外H2 O2损伤模型,采用噻唑蓝(MTT)法检测不同浓度黄芩苷对SHSY5Y细胞存活率的影响,采用Real-time PCR法检测各组细胞的Bcl-2和Bcl-xL表达水平的变化.结果 50,100,200 μmol·L-1黄芩苷组细胞存活率分别为130.4％,89.9％和60.9％,H2O2损伤组细胞存活率为33.9％,50,100 μmol·L-1黄芩苷组与H2O2损伤组比较,差异有统计学意义(P＜0.01).50,100,200 μmol·L-1黄芩苷组Bcl-2mRNA表达量分别为(11.48±0.48),(7.37±1.57),(7.39±2.01),H2O2损伤组为(5.84±0.58)；50,100,200 μmol ·L-1黄芩苷组Bcl-xL mRNA表达量分别为(19.96±2.22),(11.36±3.94),(13.07±2.37),H2 O2损伤组为(7.95±0.58),50 μmol·L-1组Bcl-2和Bcl-xL mRNA与H2O2损伤组比较,差异均有统计学意义(均P＜0.05).结论 黄芩苷对H2O2诱导的SH-SYSY细胞损伤的保护作用,可能与黄芩苷上调Bcl-2和Bcl-xL的表达而起到抗凋亡的作用有关.%Objective To study the effects of baicalin on H2O2-induced SH-SY5Y cell injury and Bcl-2 and Bcl-xL mRNA expression. Methods The H2O2-induced SH-SY5Y cell injury was established on SH-SY5Y neuroblastoma cell line in vitro. MTT assay was used to detect the proliferation of SH-SY5Y cells under different concentrations of baicalin, and Bcl-2 and Bcl-xL mRNA expression were measured by Real-time quantitative PCR. Results The survival rates of cells were 130.4% , 89.9% and 60. 9% by baicalin at 50, 100,200 μmol·L-1 , respectively, the two low dose of which showed significantly difference compared with the H2 O2 injury model group which was 33. 9% ( P<0. 01 ). Bcl-2 mRNA expression levels were (11.48±0.48),(7.37±1.57),(7.39±2.01), and(5. 84±0. 58); Bcl-xL mRNA expressions were( 19. 96±2. 22), (11. 36± 3.94),(13.07±2.37), and (7. 95+0. 58) in the group of 50,100,200 μmol·L-1 of baicalin and H2O2 injury control one, respectively. The conspicuous difference was found on Bcl-2 and Bcl-xL mRNA expression between the H2O2 injury group and baicalin at 50 μmol·L-1(P<0.01). Conclusion Baicalin can protect SH-SY5Y cell from H2O2 injury, the mechanism of which may be associated with Bcl-2 and Bcl-xL up-regulation resulting in anti-apoptosis effects.
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