姜黄素调控乳腺癌细胞增殖与转移机制研究

摘要

Objective To investigate the influence of curcumin on hypoxia-inducible factor R1, α subunit ( HIF-1α) and cell proliferation and migration of the MDA-MB-453 cell line.Methods The effects of curcumin on MDA-MEM53 cell growth was studied by means of MTT and cell count assay, cell migration analysis was determined by a transwell assay.After cells were treated with curcumin under normoxia and hypoxia, the mRNA and protein levels of HIF-1α were measured by Real-time PCR and Western blot, while the mRNA and protein levels of vascular endothelial growth factor (VEGF) were measured by Realtime PCR and ELISA, respectively.Results Curcumin inhibited cell growth and migration of the MDA-MB-453 cell line.Being treated with curcumin, either normoxia or hypoxia, the mRNA level of HIF-1α was unchange, while the protein level of HIF-1α was significantly decreased.Both the mRNA and protein levels of VEGF were obviously down-regulated.Conclusion Curcumin regulates the proliferation and migration of MDA-MB-453 cells through HIF-1α.%目的 研究姜黄素对人乳腺癌细胞MDA-MB-453中缺氧诱导因子-1α(HIF-1α)的影响及其对肿瘤细胞增殖和转移的作用.方法 采用噻唑蓝(MTT)法和细胞计数观察姜黄素抑制MDA-MB-453细胞增生作用;采用transwell侵袭小室测定姜黄素对MDA-MB-453转移的影响;采用Real-time PCR和Western blot印迹法检测常氧和乏氧状态下姜黄素对MDA-MB-453细胞系中HIF-1α的mRNA和蛋白表达水平的影响,同时采用Real-time PCR和ELISA法检测姜黄素对HIF-1α下游靶基因血管内皮生长因子(VEGF)mRNA水平和蛋白表达水平的影响.结果 姜黄素对MDA-MB-453细胞体外生长和转移具有抑制作用;常氧、乏氧状态下加入姜黄素后,MDA-MB-453细胞系中HIF-1α的mRNA水平无变化,但蛋白水平明显降低,VEGF mRNA水平和蛋白水平均明显降低.结论 姜黄素通过HIF-1α调控MDA-MB-453细胞系增殖和转移.