竹叶柴胡与北柴胡的抗炎保肝作用比较

摘要

Objective To compare anti-inflammatory and hepatoprotective effects of Bupleurum marginatum Wall. ex DC. and Bupleurum chinense DC. Methods The anti-inflammatory effect of two kinds of radix bupleuri decoction were compared using the xylene-induced ear edema mice model and the egg white-induced foot swelling rats model. Acute liver injury in mice was induced through intraperitoneal injection of peanut oil with 0. 1%carbon tetrachloride. Alanine aminotransferase ( ALT ) , aspartate aminotransferase ( AST ) , and alkaline phosphatase ( ALP ) in serum, superoxide dismutase ( SOD ) , malondialdehyde ( MDA ) , and glutathione ( GSH ) in liver tissue homogenate were detected. Histopathological changes, transforming growth factor-β ( TGF-β) and nuclear factor-κB ( NF-κB) in hepatic tissues were also observed. Results Ear swelling experiment showed that the decoction of both Bupleurum marginatum Wall. ex DC. and Bupleurum chinense DC. had equal anti-inflammatory effects (P>0. 05). Similarly,the decoction of Bupleurum marginatum Wall. ex DC. and Bupleurum chinense DC. improved levels of ALT,AST,SOD,GSH,and MDA to the same extent compared with the model control group ( P 0. 05 ). Histopathological and immunohistochemical experiments showed that expressions of TGF-β and NF- κB were similar between the two kinds of radix bupleuri. Conclusion Bupleurum marginatum Wall. ex DC. and Bupleurum chinense DC. have similar anti-inflammatory and hepatoprotective effects.%目的：比较竹叶柴胡与北柴胡抗炎、保肝作用的差异。方法采用二甲苯致耳肿胀小鼠模型及蛋清致足肿胀大鼠模型比较两种柴胡水煎液的抗炎作用；采用腹腔注射含0.1%四氯化碳( CCl4)的花生油溶液复制小鼠急性肝损伤模型，检测各组小鼠血清丙氨酸氨基转移酶( ALT)、天冬氨酸氨基转移酶( AST)、碱性磷酸酶( ALP)，肝组织匀浆超氧化物歧化酶( SOD)、丙二醛( MDA)、谷胱甘肽( GSH)；观察肝组织病理学变化及转化生长因子β( TGF-β)、核因子-κB ( NF-κB)变化。结果耳肿胀实验表明竹叶柴胡和北柴胡的水煎液均具有明显的抗炎作用，且两者差异无统计学意义(P>0.05)；竹叶柴胡组和北柴胡组ALT、AST、SOD、GSH及MDA均较模型对照组改善(P0.05)。病理切片及免疫组化结果显示，竹叶柴胡组和北柴胡组TGF-β和NF-κB表达相近。结论竹叶柴胡和北柴胡的抗炎、保肝作用相近。