高效液相色谱法测定接骨木茎叶果实中芦丁的含量

摘要

Objective To determine the content of rutin in fruits,stems,and leaves of Sambucus williamsii Hance,and provide an essential reference for the exploitation and utilization of the resources. Methods The content of rutin was analyzed on an Agilent ZORBAX Eclispse XDB C18 column(150 mmí4. 6 mm,5 μm). The mobile phase was methanol-0. 1%phosphoric acid (4555),the column temperature was set at 25 ℃,the flow rate was 0. 8 mL·min-1,and the detection wavelength was set at 360 nm. Results The linear range of rutin was 0. 105-0. 525 μg (r=0. 999 6),the average recovery was 97. 86%,and RSD was 0. 49%. The content of rutin in stems, leaves, and fruits of Sambucus williamsii Hance was 0. 171, 0. 522, and 0. 165 mg·g-1 ,respectively. Conclusion The stems,leaves and fruits of Sambucus williamsii Hance all contain rutin,but at a significant different content level. The leaves contain the highest level of rutin.%目的：测定接骨木茎、叶、果实等不同部位中芦丁的含量,为接骨木资源的合理开发和综合利用提供依据。方法采用Agilent ZORBAX Eclispse XDB C18(150 mm×4.6 mm,5μm)色谱柱,甲醇-0.1%磷酸水溶液(4555),柱温25℃,流速为0.8 mL·min-1,检测波长为360 nm。结果芦丁在0.105~0.525μg 范围内呈良好的线性关系( r=0.9996),平均回收率97.86%,RSD=0.49%。测得接骨木茎、叶、果实中芦丁含量分别为0.171,0.522,0.165 mg·g-1。结论接骨木茎、叶、果实中均含有芦丁,但差异较大,以叶中芦丁含量最高。