气候及地理环境对不同采收期广金钱草质量的影响

摘要

Objective To study the influence of climate, geographical environment and harvesting time on contents of schaftoside in Desmodium styracifolium Herba. Methods Content of schaftoside in Desmodium styracifolium Herba was determined by using HPLC. ODS-C18 column (4. 6 mmí250 mm,5 μm) was used, the mobile phase was methanol-water (3268), the detection wavelength was 272 nm, the flow rate was 0. 5 mL·min-1, and the column temperature was 35℃. Results The climate type, amount of precipitation, average temperature, duration of sunshine, geographical environment of different province had significant impacts on the content of schaftoside in Desmodium styracifolium Herba. Conclusion Subtropical monsoon climate, temperature from 25 ℃ to 32 ℃, sunshine time of 10 h per day and the average annual rainfall of 1 942 mm are suitable for growth of Desmodium styracifolium. The content of schaftoside in samples cultivated from Guangxi Province is higher than that cultivated from Guangdong Province and Hainan Province. The schaftoside content of sample cultivated in July is higher than that cultivated in other months.%目的探讨气候、地理环境及采收期对广金钱草药材中夏佛塔苷的含量的影响,为广金钱草的规范化种植提供参考。方法采用高效液相色谱法测定广金钱草药材中夏佛塔苷的含量,色谱柱为ODS-C18(4.6 mm×250 mm,5μm)。流动相为甲醇-水(3268),流速为0.5 mL·min-1,柱温35℃,检测波长272 nm。结果气候类型、降水量、年平均温度、年平均日照时间、地理环境等对广金钱草中夏佛塔苷含量有明显的影响。结论亚热带季风气候,温度在25~32℃,日照时间约10 h,平均降雨量约1942 mm最适宜广金钱草生长,广西栽培种中总含量最高,海南样品中含量最低。7月份采收的样品含量较其他月份高。