优选关木通碱制工艺中径向基函数与响应曲面法的应用

摘要

目的：优化关木通碱制工艺条件,为其炮制减毒工艺提供参考。方法采用人工神经网络与响应曲面法相结合,分析碳酸氢钠( NaHCO3)溶液、浓度、炮制时间及炮制次数对关木通中马兜铃酸的影响。结果经研究各种影响因素,得到最佳实验条件是NaHCO3浓度0.05 mol·L-1,浸泡3次,浸泡24 h,理论马兜铃总酸去除率可达83.74%。结论径向基人工神经网络与响应曲面法结合为进一步研究关木通的炮制减毒工艺提供了新思路和新方法,具有良好的指导意义。%Objective To optimize the processing conditions of Manchuiran Dutchmanspipe Stem with alkali. Methods The combination of radial basis function ( RBF) and response surface methodology ( RSM) was used to investigate the influence of NaHCO3 , concentration, duration and cycles of processing on the content of aristolochic acid. Results The optimal process was achieved when Manchuiran Dutchmanspipe Stem was soaked for 3 cycles in 0. 05 mol·L-1 NaHCO3 solution, for 24 hours in each cycle. The removal rate of total aristolochic acid approached to 83. 74%. Conclusion The combination of RBF and RSM provided a new method and good guidance for further toxicity attenuation for Manchuiran Dutchmanspipe Stem.