莲子心中酚性与非酚性生物碱体外抗氧化活性比较

摘要

Objective To compare the antioxidant activity between phenolic and nonphenolic alkaloids in Nelumbo Nucifera Gaertn.in vitro. Methods DPPH, ABTS, hydroxyl radical scavenging, super oxygen anion from oxidation, reducing power and beta carotene bleaching test methods were used to evaluate the antioxidant activity of the alkaloids. Results Half maximalinhibitory concentration ( IC50 ) of DPPH among total alkaloid, phenolic alkaloids and nonphenolic alkaloids was 21.89, 27.10 and 32.87 μg·mL-1 , respectively;IC50 of ABTS free radicals was 14.25, 20.55, 25.94μg·mL-1;The hydroxyl radical scavenging IC50 was 0.03, 0.03, 0.08 μg·mL-1; The auto-oxidation rate of super oxygen was 8.72×10-4, 5.87×10-4, 6.68× 10-4;The total alkaloid had the best reducing power, while the non-phennolic alakloids had the worst; Lipid inhibition rate of three alkaloids were 89.63%, 85.85% and 83.78% respectively. Conclusion Phenolic alkaloids are better than non-phenolic alkaloids in hydroxyl radical scavenging, reducing power and anti-lipid peroxidation, resulting in a promising prospect.%目的 比较莲子心中酚性生物碱与非酚性生物碱的体外抗氧化活性. 方法 采用1,1-二苯基-2-三硝基苯肼(DPPH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)-二铵盐(ABTS)、羟基自由基清除、超氧阴离子自氧化,还原力及β-胡萝卜素漂白测试等方法评估莲子心中生物碱的抗氧化活性. 结果 总生物碱、酚性生物碱、非酚性生物碱对DPPH自由基的半数清除浓度( IC50 )分别为21.89, 27. 10, 32. 87 μg · mL-1;对 ABTS 自由基的 IC50分别为 14. 25, 20. 55, 25.94 μg·mL-1;对羟基自由基清除IC50分别为0.03,0.03,0.08 μg·mL-1;超氧阴离子自氧化速率分别为8.72×10-4, 5.87×10-4,6.68×10-4;还原力大小:总生物碱>酚性生物碱>非酚性生物碱;脂质抑制率分别为89.63%,85.85%,83.78%.结论 莲子心中酚性生物碱对自由基的清除活性、还原力、抗脂质过氧化活性强于非酚性生物碱,具有广阔的应用前景.