星点设计-效应面法优化黔产金樱子多糖超声提取工艺∗

摘要

Objective To select the optimum ultrasonic extracting method of polysaccharides from Rosa Laevigatae Michx in Guizhou. Methods Independent variables were solid-liquid ratio, extraction time and extraction temperature, dependent variable was extraction rate ratio of polysaccharides from Rosa Laevigatae Michx.Linear or nonlinear mathematic models were used to estimate the relationship between independent and dependent variables. Response surface methodology was used to optimize the process of extraction.Prediction was carried out through comparing the observed and predicted value. Results The optimum extraction condition for the polysaccharides was as follows: extracting at 75 ℃ for 60 min and ratio of liquid to solid 30��1 ( mL��g ) . The average extraction yield of polysaccharides was 37. 54% under the optimum extraction condition. Conclusion This optimized extraction process is simple and reliable for the extraction of polysaccharides from Rosa Laevigatae Michx in Guizhou.%目的：优化黔产金樱子多糖的超声提取工艺和条件。方法以液料比、提取时间和提取温度为自变量，以黔产金樱子多糖的提取率为因变量，对自变量各水平进行多元线性回归和二项式拟合，采用效应面法优选工艺条件，并进行预测分析。结果黔产金樱子多糖优选工艺：液料比30��1( mL��g)，提取时间60 min，提取温度75℃，平均提取率37.54％。结论优化的超声提取工艺和条件简便可靠。