Optimization of Ultrasound-assisted Extraction of Lentinula Edodes Polysaccharides Using Response Surface Methodology

摘要

In this study, an ultrasound-assisted procedure for the extraction of Lentinula Edodes polysaccharides was established. Response Surface Methodology (RSM) was used to optimize the ultrasound-assisted extraction parameters (extraction temperature (A), extraction time (B) and ratio of water to raw material (C)) for enhancing the forward extraction ef?ciency of Lentinula Edodes polysaccharides by implementing a three-level, three-variable Box-Behnken experimental design. The independent variable with the largest effect on response was A (extraction temperature), followed by C (ratio of water to raw material) and B (extraction time). The optimum extraction conditions were found to be extraction temperature 76.6°C, extraction time 88.8 min and ratio of water to raw material 58.1 (mL/g). Under these conditions, theory of extraction yield of 3.83%.