金樱子黄酮与金樱子多糖对TRP V5在IgA肾病肾脏组织中表达的影响∗

摘要

Objective To investigate the effects of Rosa Laevigata Michx Flavoid( RLMF) and Rosa laevigata Michx Polysaccharose (RLMP) on expression of TRPV5 in IgA Nephropathy (IgAN) rat renal tissue. Methods Forty Sprague-Dawley rats were randomly assigned to four groups.The rat model of IgA nephropathy was induced by intragastric administration of bovine serumalbumin and injections of LPS and CC14.Eight weeks later,the rats with IgAN were treated with RLMF or RLMP (4 weeks), or normal saline.Rats was sacrificed at thirteenth weeks, and RNA was extracted from the kidney.Expression of TRPV5 in tubulointerstitial tissues were analyzed by fluorescent quantitative RT-PCR. Results After RFLP intervention,the expression levels of TRPV5 were markedly increased (P0.05),均低于正常对照组(P<0.05)；金樱子多糖组大鼠肾脏TRPV5表达显著高于模型对照组(P<0.01),与正常对照组差异无统计学意义。结论金樱子多糖可通过调节TRPV5蛋白表达调节钙离子重吸收,发挥改善肾功能作用。