Effects of Tonifying Kidney and Spleen, Detoxification of Throat for Th1/Th2 of IgA Nephropathy Model Rats

摘要

Purposes:to investigate the effects of tonifying kidney and spleen, detoxification of throat for Th1/Th2 of IgA nephropathy model rats. Methods: 72 rats were randomly divided into blank group, model group, Tripterygium wilfordii multi glycoside tablet group (control group), tonifying kidney and spleen, detoxification of throat the medicine low, middle and high dose group, referring to reports of Huiyu Lu, such as the method of preparation of animal model, the fluorescent light semi quantitative method for detection of rat renal tissue membrane IgA deposition, enzyme linked immunosorbent assays (ELISA) for detection of rate xpression level of serum cytokines interleuk in prime (IL) IL-2, IL-4, IL-10, interference prime (INF) -γ. Results:After the model results showed varying degrees of poor appetite, hair messy, weight loss, kidney mesangial IgA deposition. The treatment group rat IgA deposition has been reduced. Compared with the blank group, the model group rats serum IL-2 and INF-γ levels were significantly lower (P<0.05) and IL-4, IL-10 levels were significantly increased (P<0.05) and Thl/Th2 ratio appears to drift. Compared with model group, the serum levels of the treatment group IL-2 and INF-γ levels significantly increased (P<0.05) and IL-4, IL-10 levels decreased significantly (P<0.05) and Th1/Th2 drift improved. The traditional Chinese medicine group and the control group had no significant difference (P>0.05). Conclusion:IgAN model rat cytokine IL-2, IL-4, IL-10, INF-γ expression disorder and Th1/Th2 drift and the method of tonifying kidney and spleen, detoxification of throat can adjust the expression levels of cytokines IL-2, IL-4, IL-10 and INF-γ, and correct the Th2/Th1 ratio drift.