柴葛疏表颗粒药效学研究

摘要

Objective To study the antipyretic,anti-inflammatory and analgesic effects of Chaigeshubiao granules. Methods Animals were randomly divided into 6 groups:blank control group,indomethacin group,Fengreganmao granules group and the high, the medium and the low dosage groups of Chaigeshubiao granules ( 26, 13, 6. 5 g . kg-1 ) . Each group was administered via intragastric administration once a day for 5 days.Rat model suffering from fever by dried yeast,relieving fever of Chaigeshubiao granules was investigated;Rats with toe swelling by 1% carrageenin and mice with ear swelling by dimethylbenzene were applied to observe anti-inflammatory effects of Chaigeshubiao granules were observed;The pain models induced by 0. 6%acetic acid and the hot-plate tests in mice were used to observe the analgesic effects of Chaigeshubiao granules. Results Compared with negative control group,Chaigeshubiao granules in high dosage could obviously decrease the temperature of rat with fever induced by dried yeast 0.5 h later,and the medium dosage group decreased 4-6 h later. Moreover,Chaigeshubiao granules in high dosage could inhibit inflammatory reaction of rat with toe swelling caused by albumen at 1 h,2 h,3 h,when the medium dosage group inhibited at 2 h,3 h,4 h. The inhibition ratio of the mice with ear swelling induced by dimethylbenzene was 58.2%, 52.0% and 53.9%,respectively at the high,medium and low dose groups. And the inhibition ratio of retortion of mice by 0.6%acetic acid was 50.5%,68.8%,58.1%,respectively, at the high,medium and low dose groups. In addition,the high,medium and low dose groups reduced the pain reaction latency of mice in the hot-plate tests. Conclusion Chaigeshubiao granules have antipyretic,anti-inflammatory and analgesic actions.%目的：观察柴葛疏表颗粒的解热、抗炎、镇痛作用。方法动物按体质量随机分为空白对照组、吲哚美辛组(给予吲哚美辛)、风热感冒颗粒组(给予风热感冒颗粒)及柴葛疏表颗粒大、中、小剂量组(给予相当于生药26，13，6.5 g•kg－1)，灌胃给药，连续5 d。通过酵母致大鼠发热模型观察其解热作用，通过1％角叉菜胶致大鼠足趾肿胀模型及二甲苯致小鼠耳廓肿胀模型观察其抗炎作用，通过0.6％醋酸致小鼠急性腹膜炎模型及小鼠足部接触热板观察其镇痛作用。结果与空白对照组比较，柴葛疏表颗粒大剂量组在酵母造模后0.5 h，中剂量组在酵母造模后4~6 h可抑制大鼠体温升高；柴葛疏表颗粒大剂量组在角叉菜胶造模后1，2，3 h，柴葛疏表颗粒中剂量组在角叉菜胶造模后2，3，4 h能较好抑制大鼠足趾肿胀度；柴葛疏表颗粒大、中、小剂量组对二甲苯所致小鼠耳部炎症的抑制率分别为58.2％，52.0％，53.9％；柴葛疏表颗粒大、中、小剂量组对醋酸所致小鼠扭体次数的抑制率分别50.5％，68.8％，58.1％；热板法中柴葛疏表颗粒大、中、小剂量组均可延长小鼠痛阈值。结论柴葛疏表颗粒具有较好的解热、抗炎及镇痛作用。