Objective: To study the effects of Shuluo Huaxian granule on hepatic fibrosis in rats. Methods: Rats liver fi-brosis model were conducted by immune injury (swine serum intraperitoneal injection) preparation. Continuous made 10 weeks after treatment mode in rats, Shuluo Huaxian granule (large, medium and small three dose) was administered intragastrically for 10 weeks, and rats behavior were recorded daily. Blood samples were collected for serum biochemical indicators, serum and liver tissue index of liver fibrosis changes, and liver tissue pathology. Results; Shuluo Huaxian granule could improved rats behavior generally compared with model group. Serum alanine amino shift enzyme (ALT) , aspartate amino shift enzyme ( AST) , alkaline phosphatase (ALP) and gamma - glutamine acyl shift enzyme (GGT) in Shuluo Huaxian granule treated ( large, medium and small three dose) groups was significantly lower than model group ( P ＜ 0.01 or P ＜ 0. 05 ) . Total bilirubin ( Tbil) in high-dose group decreased obviously compared with model group ( P ＜ 0.05 ) . Precursor 1 collagen (PC ffi ), Ⅳ collagen ( Ⅳ-C) , hyaluronic acid (HA) and layer mucin (LN) of serum and liver tissue in three-dose groups was significantly lower than model group (P ＜ 0. 01 or P ＜ 0. 05 ) . Collagen fiber of liver tissue in big and middle dose groups decreased obviously than model group (P ＜ 0. 05) . Conclusion; Shuluo Huaxian granule can efficiently inhibit hepatic fibrosis on the immune injury rats.%观察中药疏络化纤颗粒对大鼠肝纤维化的影响.方法:采用免疫损伤(猪血清腹腔注射)制备大鼠的肝纤维化模型.连续造模10周后,治疗组大鼠以疏络化纤颗粒大、中、小3种剂量灌胃给药10周,观察大鼠一般情况、血清生化指标、血清及肝脏组织肝纤维化指标变化,以及肝脏组织病理学变化.结果:与模型组比较,疏络化纤大、中、小剂量组大鼠一般情况好转,血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)和γ-谷氨酰基转移酶(GGT)均显著降低(P＜0.01或P＜0.05),大剂量组大鼠总胆红素(TBil)明显降低(P＜0.05).疏络化纤各剂量组大鼠血清和肝脏中Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(Ⅳ-C)、透明质酸(HA)、层粘蛋白(LN)均显著降低(P＜0.01或P＜0.05),大、中剂量组大鼠肝组织内胶原纤维明显降低(P＜0.05).结论:疏络化纤颗粒对免疫损伤大鼠肝纤维化有明显的治疗作用.
展开▼
