姜黄素对非小细胞肺癌A549细胞的影响

摘要

Objective To study the inhibitory effect of curcumin on the proliferation,migration and invasion of non-small cell lung cancer cell A549,and to discuss further if it is closely related to the expression of c-Jun N-terminal kinase (JNK) and relative protein p38.Methods A549 cells were cultured by conventional method,and then treated with different concentration of curcumin (10,20,40,80 μmol · L-1).The proliferation,migration and invasion of A549 cells were measured by real-time cellular analysis (RTCA).The expression levels of JNK,p-JNK,p38 and P-p38 were detected by real-time PCR and Western blotting.Results Curcumin showed an antiproliferation effect against A549 cells with IC50 =40 μmol · L-1,and curcumin exhibited obviously inhibitory effect on the migration and invasion of A549 cells.Additionally,compared with control group,curcumin suppressed the expression of JNK and p38 at the gene level,and significantly inhibited the expression of JNK,P-JNK,p38 and p38 (P＜0.05) at the protein level.Conclusion These results demonstrated that curcumin can inhibit the proliferation,migration and invasion of A549 cells via reducing the level of JNK,p38 phosphorylation,and blocking JNK signal transduction pathway.%目的 探讨不同浓度姜黄素对非小细胞肺癌A549细胞增殖、迁移及浸润的影响,并进一步研究c-Jun氨基末端激酶(JNK)信号通路及相关蛋白p38的表达情况,以探讨姜黄素对肺癌A549细胞作用的分子机制.方法 常规培养肺癌A549细胞,并分为对照组、不同浓度姜黄素(10,20,40,80 μmol·L-1)处理组,运用实时无标记细胞分析技术(RTCA)实时观测并评估不同浓度姜黄素对肺癌A549细胞的增殖、迁移及浸润的影响.采用荧光定量PCR和免疫印迹分别检测JNK和p38及其磷酸化的表达情况.结果 姜黄素对A549细胞的半数抑制率为40 μmol·L-1,并且对A549细胞的迁移和浸润具有明显抑制作用;与对照组比较,姜黄素在基因水平上降低了A549细胞的JNK及P-p38的水平,而在蛋白水平上则能够显著降低P-JNK、JNK及P-p38、p38的表达(P＜0.05).结论 姜黄素能够抑制A549细胞的增殖、迁移、浸润,其可能的分子学机制为通过降低JNK、p38的磷酸化进而阻断JNK信号转导通路.