盐酸纳美芬对肺缺血-再灌注损伤大鼠肺组织β-内啡肽及IL-17的影响

摘要

Objective To study the protective effect of nalmefene hydrochloride on lung ischemia-reperfusion injury and its mechanism.Methods 40 rats were randomly divided into model group,high dose of nalmefene group,low dose nalmefene group and sham operation group equally(n =10).The lung ischemia-reperfusion model was established by occlusion of the left pulmonary hilum.The intravenous injection of nalmefene (20,10 μg·kg-1) was applied at 10 minutes before occlusion of the left pulmonary hilum in the high dose of nalmefene group and the low dose of nalmefene group,respectively.The sham operation group without occlusion of the left pulmonary hilum was not given any treatment.At 2 h after reperfusion,all rats were detected arterial blood gas value and then sacrificed.The specimens from the upper lobe of the left lung tissue were preserved to observe pulmonary lesions,detect the ratio of wet / dry weight and the expressions of β-endorphin and interleukin(IL)-17.Results Compared with the model group,the value of PCO2,the degree of pulmonary lesions,the ratio of wet / dry weight and the expressions of β-endorphin and IL-17 in lung tissue were significantly decreased (P ＜ 0.01),while the value of PO2 was significantly increased (P ＜ 0.01) in the low dose of nalmefene group.Compared with the low dose of nalmefene group,thevalue of PCO2,the degree of pulmonary lesions,the ratio of wet/dry weight and the expressions of β-endorphin and IL-17 in lung tissue were significantly decreased (P ＜ 0.01),while the value of PO2was significantly increased (P ＜ 0.01) in the high dose of nalmefene group.Conclusion Nalmefene hydrochloride may prevent lung ischemia-reperfusion injury in a dose dependent manner by reducing the production of β-endorphin and inhibiting the expression of IL-17 in lung tissue.%目的 研究盐酸纳美芬对缺血-再灌注损伤肺的保护作用及其机制.方法 将40只大鼠随机分为模型对照组、盐酸纳美芬大剂量组、盐酸纳美芬小剂量组、假手术组,每组10只.除假手术组外,其他各组以左肺门阻断法建立肺缺血-再灌注模型.盐酸纳美芬大、小剂量组大鼠分别于阻断左肺门前10 min尾静脉注射盐酸纳美芬20,10 μg·kg“.假手术组不阻断肺门,不予任何治疗.各组大鼠于再灌注2h后检测动脉血气值,留取左肺上叶组织观察肺组织损伤程度,检测肺组织湿/干重比值以及β内啡肽、白细胞介素17(IL-17)表达.结果 与模型对照组比较,盐酸纳美芬小剂量组动脉血二氧化碳分压(PCO2)、肺组织损伤程度、湿/干重比值以及β-内啡肽、IL-17表达均显著降低(P＜0.01),动脉血氧分压(PO2)显著升高(P＜0.01).与盐酸纳美芬小剂量组比较,盐酸纳美芬大剂量组动脉血PCO2、肺组织损伤程度、湿/干重比值以及β-内啡肽、IL-17表达均显著降低(P＜0.01),动脉血PO2显著升高(P＜0.0l).结论 盐酸纳关芬可通过减少肺组织内β-内啡肽生成、抑制IL-17表达而发挥预防肺缺血-再灌注损伤作用,其作用呈剂量依赖性.