独正杆有效部位促骨折愈合作用

摘要

目的 探讨独正杆有效部位促进骨折愈合的作用.方法 将SD大鼠随机分为模型对照组、云南白药组、有效部位组,每组36只.制备大鼠左前肢桡骨骨折模型.云南白药组和有效部位组分别灌胃给予云南白药胶囊内容物、独正杆有效部位3.0 g?kg-1?d-1,模型对照组给予等体积纯化水.进行血清碱性磷酸酶(AKP)及钙浓度测定、骨折部位DR成像、半定量RT-PCR测定血管内皮生长因子(VEGF)mRNA表达.结果 与云南白药组比较,独正杆有效部位AKP血清浓度峰值、钙浓度峰值显著偏高(P<0.01)、峰值出现时间更早,骨折愈合评分均显著偏高(P<0.01),骨折线消失时间明显提前,VEGF mRNA表达量在骨折后3周内均显著偏高(P<0.01).结论 独正杆有效部位对大鼠骨折愈合作用较强.%Objective To investigate the effect of effective fractions from Radix Vitis Davidii Foex. on fracture healing. Methods SD rats were randomly divided into model control group,Yunnan Baiyao group,and effective fraction group (n= 36 each group). Fracture models were established. Rats in the Yunnan Baiyao group and effective fraction group were given 3.0 g?kg-1?d-1 of Yunnan Baiyao and effective fraction from Radix Vitis Davidii Foex.,respectively,and rats in the model control group was treated with the same volume of pure water. Alkaline phosphatase and Ca concentration in serum were determined.For radius fracture structure,digital radiography and RT-PCR detection of VEGF mRNA expression were performed. Results The peak concentration of the alkaline phosphatase and Ca concentration in serum were significantly higher in the effective fraction group than in the Yunnan Baiyao group,and peak value appeared earlier,fracture healing score was higher, fracture line disappeared earlier,and VEGF mRNA expression was significantly higher in three weeks after the fracture (all P<0. 01) . Conclusion The effect of the effective fraction from RadixVitis Davidii Foex.on the fracture healing is strong.