高G-C含量突变型Foxo1转基因动物基因型鉴定PCR方法的建立

摘要

This paper focnsed on the high G-C content of the target genes and the DNA contamination from every step of the manipulation process make it difficult to correctly amplify the target genes by PCR frequently with either false negative or false positive results. In order to solve this matter,efforts were made to eliminate these barrages for genotyping with the mutant Foxo1 transgenic mice. The data showed that the correct genotyping result by PCR was obtained without either false negative or false positive phenomenon.%为解决因目的基因G-C(鸟嘌呤-胞嘧啶碱基对)含量过高导致的目的基因扩增困难,以及操作过程中多环节引起的DNA污染导致的假阳性问题.本研究以突变型Foxo1(Forkhead转录因子1)转基因小鼠作为对象,就高G-C含量转基因的PCR方法和多个环节避免DNA污染进行了探索,结果显示,实验中消除了PCR的假阴性和假阳性现象,得到了准确的突变型Foxo1转基因小鼠的基因型鉴定结果.