男性特发性无精症病人YRRM1基因的检测

摘要

目的： 建立对男性无精症病人YRRM1的基因缺失诊断方法。方法： 运用多聚酶链式反应对10例男性无精症或严重少精症的患者进行YRRM1基因位点缺失检测。结果： 10例发现1例YRRM1基因发生缺失，其它病例未发现。结论： YRRM1基因位点检测特异性位点的缺失和男性无精症有一定的相关性，可以对男性无精症和严重少精症进行一定的病因诊断。%Objective: In order to establish a method to detect the abnormal YRRM1 gene in azoospermia.Methods: We Collected ten azoospermia persons and got the DNA with phenol-chloroform.Using the special primer for YRRM1,the YRRM1 gene was analyzed by polymerase chain reaction and agar gel electrophoresis method.Results: No amplification band of YRRM1 gene was noted in 1 of 10 azoospermia person(10%).Conclusion: The deletion of YRRM1 gene may result in male infertility.We can use the method to detect partial pathogeny in azoospermia.