REAGENT KIT FOR DETECTING IDIOPATHIC AZOOSPERMIA-RELATED GENETIC MARKER

摘要

A reagent kit for detecting an idiopathic azoospermia-related genetic marker, the reagent kit comprising a primer pair, the genetic marker being located in the coding region of the AR gene, and the nucleotide sequence thereof being represented by SEQ ID No.1, the mutation sites of the sequence being one or more of c. 868 TC, c. 1484 GA, c. 1888 CT, c.569 CT, c. 616 AG, and c. 1149 CT; the upstream primer and downstream primer of the primer pair are respectively located on the upstream and downstream of the mutation sites. By means of large-scale sequencing, 6 new mutation sites of the AR gene have been selected from idiopathic azoospermia patients; research demonstrates that said mutations of the AR gene may cause idiopathic azoospermia, and thus, by means of using the present reagent kit to detect a mutation, diagnosis and detection of idiopathic azoospermia can be implemented.