为开发仙人掌在食品工业上的应用，尤其是仙人掌醇溶物在食品防腐和押菌上的应用，采用超声波辅助乙醇提取仙人掌醇溶物，通过正交试验对主要工艺参数进行优化，确定最佳提取工艺，并通过体外抑菌试验分析其抑菌活性。结果表明：超声波辅助乙醇提取仙人掌醇溶物的最佳工艺为粉碎度60-80目，乙醇体积分数75％，料液比1：20，超声波提取时间30min，在此工艺条件下，仙人掌醇溶物得率为15．63％；体外抑菌试验表明，仙人掌醇溶物对细菌（大肠杆菌）、霉菌（黄曲霉）和酵母菌（鲁氏酵母）等常见食品腐败菌都有良好的抑制作用。%In order to explore the application of Opuntia stricta in food industry,especially for its application in antimicrobiai activity,the main technical parameters of the ultrasonic-assisted extraction of the alcohol soluble substance from opuntia were optimized by the orthogonal experiment, and its antimicrobial activity were analyzed. The best technical parameters were as follows： fragmentation degree was 60-80 mesh,the volume fraction of ethanol was 75%, the proportion of material to liquid was 1：20, time of Ultrasonic extraction was 30 min. In this condition,the yield of alcohol soluble substance from opuntia was 15.63%. By using filter paper diffusion method,inhibitory activity of alcohol soluble substance from opuntia on food spoilage bacteria：bacteria （E. coli）, mold （Aspergillus flavus ） and yeast（Saccharomyces rouxii ） was studied, and the minimal concentration（MIC）was determined by concentration gradient method. Results indicated that alcohol soluble substance of opuntia had effective inhibition on three kinds of tested bacteria.
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