Oocyte maturation culture technology in vitro played an important role in modern biotechnology .In order to improve the quality of sheep oocytes maturation and promote research of animal biotechnology ,main influencing factors of oocyte maturation in vitro had been reviewed including season factors ,sheep age ,source of oocytes ,culture conditions ,methods ,culture medium ,adding substances and so on .%卵母细胞体外成熟培养技术是现代生物技术中的重要内容之一,为提高绵羊卵母细胞的成熟质量和促进动物生物技术研究,综述了绵羊卵母细胞体外成熟的主要影响因素,主要包括季节因素和绵羊年龄、卵母细胞的来源、培养条件和方法、成熟培养液及其它添加成分等。
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