白屈菜乙醇提取液对菜青虫体内保护酶活性的影响

摘要

In order to develop new botanical pesticides by Celandine majus ,Spectrophotometry was used to de‐termine the change of ethanol extract of Chelidonium majus on protective enzyme activity of Pieris rapae L .. The results showed that the SOD ,CAT and POD activity were presented increased and then decreased after ethanol extract treating ,which SOD enzyme activity reached maximum for 16 h ,CAT and POD reached for 24 h .It indicated that in the process of withstand environmental stress for Pieris rapae L .,SOD was a protec‐tive enzyme which worked first ,following with CAT and POD .Before 36 h ,three protective enzyme treatments were higher than those in the control ,after 48 h ,they were lower than the control .The results showed that , ethanol extract of Chelidonium maj us could effectively interfere insect protection enzymes ,disrupt the normal physiological metabolism ,so as to achieve to control .%为利用白屈菜开发新型的植物源农药，采用分光光度法测定了白屈菜乙醇提取液对菜青虫体内保护酶活性的变化。结果表明：用白屈菜乙醇提取液处理菜青虫后，其体内SOD、CAT和POD活性都呈现先升高后降低的趋势，其中SOD酶活力在16 h达到最大，而CAT、POD都在24 h达到高峰，说明菜青虫在抵御逆境胁迫过程中，SOD是首先发生作用的保护酶，CAT和POD是继SOD之后发生作用的酶系。处理36 h前，3种保护酶处理组均高于对照组，48 h后，均低于对照组。说明，白屈菜乙醇提取液能有效干扰昆虫保护酶系，扰乱其正常的生理代谢，从而起到防治效果。