垂枝樱花组培技术研究

摘要

In order to establish more efficient tissue culture system ,using the stems of Cerasus subhirtella Miq . var .pendula Tanaka as the explants ,the effects of different sterilize time ,starting culture ,proliferation culture and rooting culture were studied .The results showed that the procedures of surface sterilization was 75% et‐hand soak for 30 s ,0 .1% HgCl2 immerse for 10 min ,the sterile water rinse 5 times ,the survival rate of 66 .7% .The suitable inducing medium was :1/2 MS+6‐BA 0 .5 mg·L‐1 + NAA 0 .1 mg·L‐1 ,the differentia‐tionrate reached 68 .3% ,the proliferation medium was MS + 6‐BA 0 .3 mg·L‐1 + NAA 0 .05 mg·L‐1 + GA3 10 mg·L‐1 ,and the proliferation coefficient was 7 .0 ,the average height was 2 .9 cm ,the root inducing medium was 1/2 MS+NAA 1 .0 mg·L‐1 +IBA 0 .5 mg·L‐1 +20 g·L‐1 sucrose and the rooting rate could reach 93% .%为了建立更高效的垂枝樱花的组织快繁体系，以垂枝樱花的当年生带腋芽茎段为外植体，进行了外植体的灭菌、启动、增殖和生根培养，探讨了适宜的外植体灭菌方法与程序，不同培养基配方对启动、增殖、生根培养效果的影响。结果表明：适宜的表面灭菌方法为75％乙醇浸泡30 s ，0．1％的HgCl2浸泡10 min ，最后无菌水冲洗5遍，成活率达66．7％；较适宜的启动培养基为1／2 MS＋6‐BA 0．5 mg·L‐1＋ NAA 0．1 mg·L‐1，分化达68．3％；适宜的增殖培养基为：MS＋6‐BA 0．3 mg·L‐1＋NAA 0．05 mg·L‐1＋GA310 mg·L‐1，增殖系数为7．0，平均苗高2．9 cm ；适宜的生根培养基为：1／2 MS＋NAA 1．0 mg·L‐1＋IBA 0．5 mg·L‐1＋蔗糖20 g·L‐1，生根率达93％。